|
KMID : 0545119930030020091
|
|
Journal of Microbiology and Biotechnology
1993 Volume.3 No. 2 p.91 ~ p.94
|
|
|
Nucleotide Sequencing Analysis of a Gene Coding for 3-Isopropylmalate Dehydrogenase of Kluyveromyces fragilis
|
|
HONG, SOON DUCK
KIM, JONG GUK/LEE, DONG SUN/WOO, JU HYUNG/LEE, SANG YONG
|
|
|
Abstract
|
|
|
|
A 3-isopropylmalate dehydrogenase (3-IPMD) gene was cloned from Kluyveromyces fragilis. pJK104 could complement Escherichia coli leuB and Saccharomyces cerevisiae leu2 auxotrophs. The coding region was subcloned and the nucleotide sequence was determined. A 1.8 Kb EcoRI/SphI fragment of pJK104 subcloned in pUC18 could still complement the leuB mutation. An open reading frame of 1164 by that corresponds to a polypeptide of 387 amino acids was found in the cloned fragment. The homology between the 3-isopropylmalate dehydrogenase of S. cerevisiae and that of K. fragilis was 68.13% in nucleotides.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|